FV1200 - Key Features


Precise, Bright Imaging with Minimum Phototoxicity

The FLUOVIEW FV1200 offers excellent precision, enhancing sensitivity and stability with an advanced optical system, scanners and detection system. In combination with the IX83 microscope, the FV1200 provides users with new opportunities in live cell imaging.

  • Upright Frame
The FV1200 upright frame is a dedicated solution to perform three-dimensional fluorescence imaging for the specimen including the tissue section.
  • Inverted Frame
The FV1200 inverted frame solution is dedicated to perform Live Cell Imaging. It's high rigidity structure significantly reduces the impact of heat and vibration and improves the quality of time-lapse imaging. Integration with the IX3-ZDC Z drift compensator enables imaging without focus drift or misalignment.
  • Laser Systems

The multi-combiner enables combinations with all of the following diode lasers: 405 nm, 440 nm, 473 nm, 559 nm and 635 nm. Also be equipped with conventional Multi-line Ar laser and HeNe (G) laser.

  • Dual Type
The multi-combiner outputs laser light with two fibers. Light can be used both for observation and photostimulation.
  • Single Type
Single channel laser output. AOTF is standard equipment. 
  • Choice of Main Scanner

Select the scanner to match the purpose at hand, with a choice of a spectral scan unit that achieves 2 nm resolution for high-precision spectroscopy, and a filter scan unit incorporating high-quality filters.

  • High-performance Detection System
High-performance, high S/N ratio and superior optical quality are achieved through the smooth integration of a pupil projection lens, a high performance photomultiplier tube, silver-coated galvanometer scanning mirrors with high reflectance across a broad range of wavelengths, and an analog processing circuit that reduces noise to an absolute minimum. 
  • High-sensitivity Detector

A high-sensitivity detector employing gallium phosphide (GaAsP) is also available as an option.

  • Dedicated Scanner for Photostimulation

Combining the main scanner with a photostimulation scanner provides essential flexibility for tracking the diffusion or transport of fluorescently labeled molecules, or for marking specific live cells.

  • UIS2 Objectives

Olympus UIS2 objectives offer world-leading, infinity-corrected optics that deliver unsurpassed optical performance over a wide range of wavelengths.

  • High S/N Ratio Objectives with Suppressed Autofluorescence

Olympus offers a line of high numerical aperture objectives with improved fluorescence S/N ratio, including objectives with silicone immersion, exceptional correction for chromatic aberration, total internal reflection fluorescence (TIRF), and oil- and water immersion objectives.


Precision and Reliability Meets Deep 3D Imaging

  • High-resolution Silicone Immersion Objectives
Silicone immersion objectives can be designed with a larger numerical aperture (NA) than water immersion objectives, increasing image resolution and brightness. Discover continuous high-resolution observation during extended time-lapse imaging.
  • Acquire and Analyze Colocalization Imaging with the PLAPON60XOSC
This oil immersion objective minimizes lateral and axial chromatic aberration in the 405-650 nm spectrum, while supporting the reliable acquisition and measurement of colocalization images with superior positional accuracy.
  • The IX3-ZDC Z Drift Compensator Offers a Range of Functionality for Autofocus
The IX3-ZDC uses low phototoxicity IR light to detect the correct focus position as set by the user. One-shot AF mode allows several focus positions to be set as desired for deeper samples, enabling efficient Z-stack acquisition in multiposition experiments. Continuous AF mode keeps the desired plane of observation precisely in focus, avoiding focus drift caused by temperature changes due to perfusion or reagent addition.
  • ZDC One-shot Function Detects Focus Fast, Even in High Magnification Observation

IX3-ZDC focus detection and tracking can be performed via the innovative touch panel, independent of software.

  • Tackle the Conflicting Requirements of Expandability and Rigidity with the IX3
A Z-drive guide installed near the revolving nosepiece combines high thermal rigidity with the further stability of a wraparound structure to significantly reduce the impact of heat and vibration and improve the quality of time-lapse imaging.Furthermore, combination with a motorized stage that enables multi point registration allows the achievement of high precision multi point time-lapse imaging.


Olympus's Unique Super Resolution Technology

  • FV-OSR (Olympus Super Resolution) technology

Olympus’ widely applicable super resolution method requires no special fluorophores, and can work for a wide range of samples in combination with a large selection of superior optics and high sensitivity detectors. Ideal for colocalization analysis, sequential or simultaneous acquisition of 2 fluorescent signal.

 

User-Friendly Software

  • Configurable Emission Wavelength

Emission wavelength can be selected by a choice of the dyne name to set the optimal filters and laser lines.

  • Wide Choice of Scanning Modes

Diverse scanning modes including ROI, point and high-speed bidirectional scanning can be set by the easy control window.

  • Adjustable Excitation Laser Power

Easily adjust the optimum laser power for each specimen (live cells and fixed specimens).

  • Image Acquisition by Application

User-friendly icons offer quick access to functions, for image acquisition according to the application (XYZ, XYT, XYZT, XYλ, XYλT).

  • Time Controller
Precisely synchronizes different experimental protocols including FRAP, FLIP and FRET by acceptor photobleaching and time-lapse. Save and open settings for later use.
  • Dark Application Skin

The Dark Application Skin minimizes the influence of the noise from the screen to the detecting senors by darkening the control screen.


Multi-Dimensional Time-Lapse

  • Multi-dimensional Time-lapse Imaging with Outstanding Positional Accuracy

The FLUOVIEW FV1200 can be used for ideal multi-dimensional time-lapse imaging during confocal observation, using multi-area time-lapse software to control the motorized XY stage and IX3-ZDC Z drift compensator.

Simultaneous Photostimulation

  • Combined Photostimulation and Imaging with Microsecond Precision Control

The SIM scanner system combines the main scanner with a photostimulation scanner. Control of the two independent beams enables simultaneous stimulation and imaging, to capture reactions during stimulation.

  • FLIP - Fluorescence Loss in Photobleaching
Fluorescence loss in photobleaching (FLIP) combines imaging with continuous bleaching of a specific region to observe the diffusion of a target protein within a cell. The changes in the image over time make it possible to observe the location of structural bodies that inhibit the diffusion of the molecule.
  • FRAP - Fluorescence Recovery after Photobleaching
Exposure of fluorescent-labeled target proteins to strong laser light causes their fluorescence to fade locally. Fluorescence recovery after photobleaching (FRAP) is used to observe the gradual recovery of fluorescence intensity caused by protein diffusion from the area surrounding the bleached region. By examining the resulting images, it is possible to characterize the diffusion speed of the molecule, and the speed of binding and release between the molecule and cell structures.
  • Uncaging
A 405 nm laser is optional for uncaging with the SIM scanner system. Caged compounds can be uncaged point-by-point or within a region of interest, while the main scanner of the FV1200 captures images of the response with no time delay.
  • Multi-Stimulation Software Enables High Speed Multi point Scans and Mapping Scans
Users can designate the number of points on an image for light stimulation. Stimulation timing, duration and interval can be defined in a magnitude of μs and the user can program the experiment with continuous or pulse stimulation. The same software also provides features that allow extended multiple points surrounding one single point to cover a small area.


Diffusion Measurement Package

  • Diffusion Measurement Package Extends Analytical Capabilities

This optional software module enables data acquisition and analysis to investigate the molecular interaction and concentrations by calculating the diffusion coefficients of molecules within the cell. Diverse analysis methods (RICS/ccRICS, point FCS/point FCCS and FRAP) cover a wide range of molecular sizes and speeds.


Accessory Units That Support an Array of Applications

  • Fluorescence Illumination Source/U-HGLGPS
The pre-centered fluorescence illumination source requires no adjustment and has an average lifespan of 2,000 hours.


Transmitted Light Detection Unit

External transmitted light photomultiplier detector and 100 W Halogen conventional illumination, integrated for both laser scanning and conventional transmitted light
  • 4th Channel Detector Unit
Attaches to the optional port of either the filter or spectral type scanning unit and is used as a 4th confocal fluorescence detection channel. This is a filter-based fluorescence detection unit.
  • Fiber Port for Fluorescence Output
Confocal fluorescence emission can be introduced via a fiber delivery system into an external device. The fiber port equipped with FC connector (fiber delivery system not included).
  • TIRF Unit
Enables control of the necessary volume of excitation light using FV1200 software. This unit enables TIRF imaging using the confocal laser light source.